Figure 5.

ODN2088 inhibited PANoptosis in SAE rats. At 24 h after CLP, (A) caspase-3, Bcl-2, (B) NLRP3, caspase-1, (C) RIPK1, RIPK3, p-MLKL in different groups are shown, with GAPDH used as the loading control. n = 5. Data are represented by histograms, and the sham group is normalized. ^∗P < 0.05. All experiments were repeated three times and error bars depict mean ± standard deviation. CLP: Cecal ligation and perforation; MLKL: Mixed lineage kinase domain-like protein; NLRP3: NOD-, LRR-, and pyrin domain-containing protein 3; PANoptosis: Pyroptosis, apoptosis, and necroptosis; RIPK: Receptor-interacting protein kinase; SAE: Sepsis associated encephalopathy.