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. 2022 Aug 9;41(38):4336–4348. doi: 10.1038/s41388-022-02426-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A PURα-interacting proteins were identified in KYSE510 cells using a coimmunoprecipitation assay coupled to a label-free quantification (LFQ) proteomic technique. The x-axis indicates the log2-transformed LFQ intensity ratios, and the y-axis indicates the log10-transformed total intensities. The 494 PURα-interacting proteins (red dots) that met the following screening criteria (unique peptides ≥1 and LFQ intensity ratio ≥1.8) are shown. B AVenn diagram between PURα-interacting proteins and the proteome of stress granules and P-bodies was constructed. C GO analysis was implemented with DAVID based on PURα-interacting proteins. The top 10 enriched pathways are shown. D The interaction between PURα and candidate representative proteins associated with mRNA translation was verified by coimmunoprecipitation in KYSE510 cells. E The interaction between PURα and PABPC1 and between PURα and EIF3B was visualized via immunofluorescence staining in KYSE510 cells separately. Scale bars: 30 μm. F The interaction between PURα and PABPC1, and between PURα and EIF3B was visualized via immunofluorescence staining in ESCC tissue separately. Scale bars: 100 μm. G PURα-Flag and PABPC1-HA were overexpressed in 293 cells separately, and the interaction between exogenous PURα and PABPC1 was detected by a coimmunoprecipitation assay.