Extended Data Fig. 6. Correct cell sorting and self-organization is necessary for proper morphogenesis.
(a) Comparison and quantification of cavity formation in structures containing mis-sorted ES or TS and (b) mis-sorted XEN cells. Well-sorted structures: n = 73; Mis-sorted ES structures: n = 103; Mis-sorted TS structures: n = 109. Mis-sorted XEN structures: n = 57. N = 3 for each condition. Scale bar, 100 μm. Statistics calculated by unpaired two-tailed Student’s t test. (c) The average length and (d) internal cavity size of Cadherin OE ETX and control ETX at different time points. 20 to 30 structures were collected at each time points. Data are presented as Mean ± SD. Statistics calculated by unpaired two-tailed Student’s t test. P values indicate significant difference between Cadherin OE and control ETX at the same time point. (e) Comparison and quantification of basement membrane formation in structures containing well-sorted and mis-sorted XEN. Well-sorted structures: n = 84; Mis-sorted XEN structures: n = 74. N = 3 for each condition. Data are presented as Mean ± SD. Statistics calculated by unpaired two-tailed Student’s t test. Scale bar, 100 μm. (f) Schematic image shows natural and ETX embryos use different routes to form the post-implantation embryos. In ETX embryos, lineage-specific stem cells bypass the blastocyst structure to directly assemble a post-implantation embryo. Source numerical data are available in source data.