Extended Data Fig. 2. Membrane-fusion regulator, tether, and SNAREs are required for GPAT4 targeting to LDs.
a, Depletion of specific Rab, tethering-complex components, and SNAREs abolishes GPAT4 targeting to LDs. Confocal imaging of live EGFP-GPAT4 endogenous knock-in cells upon RNAi of membrane fusion machinery components, 20 h after 1 mM oleic acid treatment (except for 0 h timepoint for LacZ RNAi). LDs were stained with MDH. Representative images are shown. Scale bar, 5 and 1 μm (inlay). Quantification of targeting ratios is shown in Fig. 3b. b, Quantitative PCR to verify RNAi. Mean ± SD, n = 3. Two-tailed Student’s t-test for each gene, *p < 0.05 (from left to right: 0.0497, 0.0241, 0.0369, 0.0258, 0.0104, 0.0180, 0.0125, 0.0140, 0.0122, 0.0264, 0.0294, 0.0271, 0.0250, 0.0462, 0.0259, 0.0430, 0.0172, 0.0104, 0.0369, 0.0142, 0.0420), **p < 0.01 (from left to right: 0.0070, 0.0076, 0.0051), ***p = 0.0001, compared to Control (LacZ) RNAi. Source numerical data are available in source data.