Fig. 2.

In-silico analyses of the CAT rs1001179 SNP region. A Schematic representation of human CAT promoter and location of the analyzed CpG sites. Red bars represent the location of the 8 CpG sites on the proximal region of human CAT promoter that have been analyzed with bisulfite conversion and pyrosequencing. The analyzed CpG sites range from ATG − 357 to − 306. This region encompasses the rs1001179 SNP. B Multiple sequence alignment of a selected upstream promoter region among 15 CAT genes belonging to primates, no-primate mammals, rodents, and zebrafish. GenBank accession references: Homo sapiens (human), NC_000011.10; Pongo abelii (orangutan), NC_036914.1; Pan troglodytes (chimpanzee), NC_036890.1; Pan paniscus (pygmychimpanzee), NC_048250.1; Gorilla gorilla (gorilla), NC_044613.1; Nomascus leucogenys (white-cheeked gibbon), NC_044395.1; Macaca mulatta (rhesus macaque), NC_041767.1; Callithrix jacchus (common marmoset), NC_048393.1; Otolemur garnettii (greatergalago), NW_003852396.1; Rattus norvegicus (brownrat), NC_005102.4; Mus musculus (house mouse), NC_000068.8; Bos taurus (cattle), NC_037342.1; Sus scrofa (wild boar), NC_010444.4; Canis lupus familiaris (dog), NC_006600.3; and Daniorerio (zebrafish), NC_007136.7. C Putative binding sites for TFs associated with the rs1001179 SNP C allele (upper panel) or with the rs1001179 SNP T allele (lower panel). GATA-1: GATA-binding factor 1; TFII-I: Transcription Factor II-I; STAT4: Signal Transducer and Activator of Transcription 4; c-Ets-1: ETS Proto-Oncogene 1; GR-β: Glucocorticoid Receptor beta