Fig. 3.

Association between CAT mRNA expression and rs1001179 SNP. A Comparison of CAT mRNA levels between the CC and CT/TT genotypes of rs1001179 SNP in CLL cells (n = 33). B Comparison of CAT mRNA expression between CC and CT genotypes for rs1001179 SNP in HD B cells (n = 10). Data are expressed as relative quantification using comparative Ct method (2^–ΔΔCt), normalized to the expression value of the human embryonic kidney 293 cell line (HEK293) set as 1, and reported as mean ± SEM. Comparisons were performed with Mann Whitney test. *P < 0.05. C ChIP assay for TFs binding to CAT promoter in primary CLL cells harboring CC (n = 5) and CT/TT (n = 7) genotypes. Cross-linked chromatin was immunoprecipitated with antibodies against ETS1, GR-β, STAT4 or Non-Immune IgG negative control (IgG). Precipitated DNA was amplified (from − 371 to − 255, human CAT promoter region location from ATG) by qPCR. Data are expressed as percentage of input DNA (un-immunoprecipitated DNA) and reported as mean ± SEM. Comparisons were performed with Kruskal–Wallis test and each P value was corrected for multiple comparisons using the Dunn’s test. **P < 0.01, ***P ≤ 0.001. CLL chronic lymphocytic leukemia, HDs healthy donors, ETS Proto-Oncogene 1 (ETS1), Glucocorticoid Receptor beta (GR-β) and Signal Transducer and Activator of Transcription 4 (STAT4)