Fig. 5.

Association between methylation levels and CAT mRNA expression levels. A Association of DNA methylation percentage of sites CpG#22 to CpG#18 with CAT mRNA expression in CLL (n = 21) and HD B cells (n = 10). B MEC1 cells (6 experimental replicates), primary CLL cells (n = 7; CC = 4, CT = 2, ND = 1), and HD B cells (n = 3) were treated with 2 μM DAC or left untreated (vehicle) for 96 h and then analyzed for CAT mRNA levels. Data are expressed as relative quantification using comparative Ct method (2^–ΔΔCt), normalized to the expression value of the human embryonic kidney 293 cell line (HEK293) set as 1, and reported as mean ± SEM of 5 independent experiments. C MEC1 cells were treated with 2 μM DAC or left untreated (vehicle) for 96 h and then analyzed for CAT protein levels. Data are expressed as fold autofluorescence calculated as median fluorescence intensity (MFI) divided by fluorescence-minus-one (FMO) and reported as mean ± SEM of 9 independent experiments. Comparisons were performed with Wilcoxon matched-pairs signed rank test. *P < 0.05. CLL chronic lymphocytic leukemia, HDs healthy donors, DAC 5-aza-2’-deoxycytidine, ND not defined for rs1001179 SNP