Table 2.

Binding affinities of compound 1 analogs reveal the importance of the triple-ring and imino group of compound 1

Compound	Structure	SMN	UHRF1
		Kd (µM)
1		2.6 ± 0.1	16 ± 1
2		9.3 ± 0.6	No binding
3		31 ± 2	No binding
4		13 ± 1	No binding
5		12 ± 1	No binding
6		12 ± 2	Weak binding
7		No binding	No binding
8		No binding	No binding
9		No binding	No binding
10		No binding	No binding
11		No binding	No binding
12		No binding	No binding
13		No binding	No binding
14		No binding	No binding
15		No binding	No binding
16		No binding	No binding
17		No binding	No binding
18		No binding	No binding
19		No binding	No binding
20		No binding	No binding

ITC data shown are representative of two independent experiments. Source data are provided as a Source Data file. K_d: binding affinity.