Table 6.

Predicted HLA class-II binding peptides in block IV of PmMSP1.

Amino acid residue†	Allele	Peptides and their variants	Prevalence (%, n = 35)	HLA§	Allele Frequency*	IC50#	Peptide rank#
207–221	I	KKEYNNIADENKKLE	11.43	None	–	–	–
	II	KKEYDNIATTNKELE	45.71	DQA1*04:01/ DQB1*04:02	0.0021/ 0.0032	747	9.9
	III	KKEYDNIATKNKELE	25.71	None	–	–	–
	IV	KKEYDNITTTNNELV	11.43	DRB1*13:02	0.0138	43.7	5.5
				DRB1*04:05	0.0489	235.4	10
	V	KKEHDNIATTNNELE	2.86	DQA1*04:01/ DQB1*04:02	0.0021/ 0.0032	478	6.4
				DQA1*03:01/ DQB1*03:02	0.0457/ 0.0426	878	9.9
	VI	KKEYDNIATTNNELK	2.86	None	–	–	–
211–225	I	NNIADENKKLEAPSE	8.57	None	–	–	–
	II	DNIATTNKELEAPSG	31.43	None	–	–	–
	III	DNIATKNKELEAHSG	22.86	None	–	–	–
	IV	DNIATTNKELEASSG	14.29	None	–	–	–
	V	DNITTTNNELVTSNV	11.43	DRB1*13:02	0.0138	55.9	6.6
	VI	DNIATTNNELEASSG	2.86	None	–	–	–
	VII	DNIATKNKELEAPSG	2.86	None	–	–	–
	VIII	DNIATTNNELKAPSE	2.86	None	–	–	–
	IX	NNIADENKKLEASSE	2.86	None	–	–	–

^†Positions and amino acid substitutions are based on the FJ824669 sequence.

^§Analysis based on the HLA alleles available in the IEDB analysis resource (accessed February 18, 2022).

*Allele frequency among Thai population⁴⁴.

^#Based on NN-align and the IEDB recommended 2.22 method⁴³.