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. 2022 Sep 16;13:5449. doi: 10.1038/s41467-022-33227-7

Table 1.

Intersubunit crosslinks identified in purified augminΔH6C complex

CL distance (Å) Protein 1 Peptide 1 Protein 2 Peptide 2 Score
1 9.6 HAUS1 60QKASEYESEAK HAUS4 73LHKTTWLR 13.2
2 13.7 HAUS1 255IEEAKRELDSIEAELTR HAUS4 301QATENKR 20.4
3 16.8 HAUS2 202NIPHLAANLKK HAUS6 330LTVDLHYLEKETK 43.3
4 10.6 HAUS2 214KMNQALAK HAUS7 335AVETVKK 28.4
5 9.1 HAUS2 241KQQNEVSSCIPK HAUS7 362MNELMEK 21.3
6 14.4 HAUS3 122CQLMASVTSHKSLR HAUS4 116ARLQQEVEEQLKKK 21.1
7 17.9 HAUS4 116ARLQQEVEEQLKKK HAUS3 121NKCQLMASVTSHK 32.4
8 19.4 HAUS5 309STLLKER HAUS2 241KQQNEVSSCIPK 21.3
9 21.7 HAUS5 77KLELEAAVTR HAUS3 67SGKPILEGAALDEALK 23.5
10 20.0 HAUS5 77KLELEAAVTR HAUS3 86TSDLKTPR 18.5
11 21.8 HAUS5 196AQFLQNLLLPQAKR HAUS3 370QELVLNQLIKQK 28.3
12 57.4 HAUS5 428KVVPTFEAVAPQSR HAUS3 339NTIDTKDYSTHR 20.4
13 a HAUS5 465HRPGELKPLPTVLPSIHQLHPASPR HAUS3 339NTIDTKDYSTHR 37.3
14 10.5 HAUS5 619LRWVQAQGALQKLCS HAUS3 596IKAVSLED 20.3
15 22.8 HAUS5 77KLELEAAVTR HAUS3 67SGKPILEGAALDEALK 23.5
16 17.0 HAUS5 309STLLKER HAUS7 362MNELMEK 26.3
17 21.1 HAUS6 367HSVVEKQGEWHKK HAUS2 241KQQNEVSSCIPK 17.4
18 51.8 HAUS6 358IKDDLTTIR HAUS3 323LEKEVTQIK 18.3
19 314.3 HAUS6 217KMEPYDDHSNMEEKIQKVR HAUS4 276QVLNSYEVLGEEFDR 20.1
20 b HAUS6 348LSDLKHMR HAUS8 60GKMSEGGR 15.7
21 11.2 HAUS6 348LSDLKHMR HAUS8 299DVTAKK 25.5
22 18.6 HAUS6 358IKDDLTTIR HAUS8 299DVTAKK 22.5
23 11.9 HAUS7 231QLQESAAKLHALR HAUS2 113NLEIELLKLEK 31.4
24 16.2 HAUS7 231QLQESAAKLHALR HAUS2 121LEKDTADVVHPFFLAQK 16.4
25 14.5 HAUS7 205QSDDWQWASASAKSEEEEKLAELAR HAUS8 173RAEKNLLIMCK 19.3
26 15.7 HAUS7 219SEEEEKLAELAR HAUS8 177NLLIMCKEK 32.4
27 18.1 HAUS7 219SEEEEKLAELAR HAUS8 185EKLQKK 18.4
28 11.7 HAUS8 299DVTAKK HAUS6 343FQKER 20.3
29 a HAUS8 356ESGGAPKNTPLSEDDNPGASSAPAQATFISPSEDFSSSSQAEVPPSLSR HAUS6 348LSDLKHMR 31.4
30 16.5 HAUS8 311SFAQVLELSAEASKEAALANQEVWEETQGMAPPSR HAUS6 358IKDDLTTIR 18.5
31 a HAUS8 356ESGGAPKNTPLSEDDNPGASSAPAQATFISPSEDFSSSSQAEVPPSLSR HAUS6 355YRIKDDLTTIR 25.3
32 a HAUS8 60GKMSEGGR HAUS7 94FSSLKGVPTEVK 17.5
33 20.1 HAUS8 311SFAQVLELSAEASKEAALANQEVWEETQGMAPPSR HAUS7 362MNELMEK 26.5

Numbers in left column correspond to labels in Fig. 3c. Crosslinked amino acids are underlined in bold. Score is the Total XL score from Metamorpheus.

aPeptide 1 is not present in structure.

bPeptide 2 is not present structure.