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. Author manuscript; available in PMC: 2023 Sep 15.
Published in final edited form as: Mol Cell. 2022 Jul 15:S1097-2765(22)00612-8. doi: 10.1016/j.molcel.2022.06.029
Primer Sequence
sgRNA_Smad3_N-terminus GTGACCCTTCGGTGCCAGCC
sgRNA_Smad7_N-terminus TAGCCGGCAAACGACTTTTC
Smad3-5HA-F1 GAGGGATCCTTAAGGGCGAATTCTGCAGATGCGGCGACTGCGCTGGGAAGGAGGCTG
Smad3-5HA-R1 GTACCGATTTCTGCCATAGCTGACAACGCAGAGTTACCGGCGCCCCC
Halo-for Smad3-F1 GTAACTCTGCGTTGTCAGCTATGGCAGAAATCGGTACTGGC
Halo-for Smad3-R1 GTGAAGGGCAGGATGGACGACATGATCGCGTTATCGCTCTGAAAGTACAG
Smad3-3HA-F1 CGATAACGCGATCATGTCGTCCATCCTGCCCTTCACCCCCCCGATC
Smad3-3HA-R1 CATGCTCGAGCGGCCGCCAGTGTGATGGATCCTCCACGAGCGCGGGGCGGGAGGCGGCGGAG
Smad7-5HA-F1 GAGGGATCCTTAAGGGCGAATTCTGCAGATTGCTTAGCAAGGGGGAAAGAGGCTTTTTCCTC
Smad7-5HA-R1 CCGATTTCTGCCATGCGGGGCGAGGAGGCGAGGATAAAATTCATTCGCAGGTTAAGGAG
halo-for Smad7-F1 CCTCGCCTCCTCGCCCCGCATGGCAGAAATCGGTACTGGC
halo-for smad7-R1 CGTTTGGTCCTGAACATGATCGCGTTATCGCTCTGAAAGTACAG
Smad7-3HA-F1 CTTTCAGAGCGATAACGCGATCATGTTCAGGACCAAACGATCTGCGCTCGTC
Smad7-3HA-R1 CATGCTCGAGCGGCCGCCAGTGTGATGGATCTCTTGACTTCCGAGGAATGCCTGAGATCC