FIG. 2.

Analysis of protein p6 in B. subtilis infected with mutant sus6(626). Polypeptides present 40 min after the infection of suppressor (su⁺) and nonsuppressor (su⁻) B. subtilis with φ29 mutants carrying nonsense mutations in gene 6 (sus6) and gene 14 (sus14) were separated by electrophoresis in a 10-to-20% gradient polyacrylamide gel in the presence of SDS. Proteins were transferred to a Millipore polyvinylidene difluoride membrane, and proteins p5, p6, and p17 were detected with a mixture of the corresponding monospecific antisera.