FIG. 4.

Primer extension analysis of the transcripts produced throughout the infection cycle in B. subtilis infected with mutant sus6(626). RNA synthesized by mutant sus6(626) in nonsuppressor (su⁻) or suppressor (su⁺) B. subtilis or by the control mutant sus14(1242) at an MOI of 5 was isolated at the time after the infection indicated at the bottom of each graph. Transcripts derived from each of the promoters (indicated above each graph) were analyzed by the extension of radioactively labeled specific primers and quantitated as described in Materials and Methods. The graphs show the amount of mRNA observed during an infection cycle, where each point represents the average of at least three independent experiments. Arbitrary units are comparable between graphs.