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. Author manuscript; available in PMC: 2023 Sep 15.
Published in final edited form as: Cell. 2022 Aug 23:S0092-8674(22)01061-3. doi: 10.1016/j.cell.2022.08.012
Scope Body Objectives Excitation Ex/Em Setup Confocal
Setup
Camera
Zeiss Axios Observer Z1 5X
20X
63X
Colibri LED (full spectrum) DAPI 365/445
GFP 470/525
YFP 550/605
mCherry
560/630
Cy5 640/690
N/A Hamamatsu Image EM-1K, Hamamatsu Orca-R2
Nikon Eclipse Ti2 4X
20X
60X
Lumencor SpectraX LED:
395nm
440nm
470nm
510nm
550nm
575nm
640nm
DAPI 395/460
CFP 440/480
GFP 470/525
YFP 510/535
mCherry
575/630
Cy5 640/700
N/A Andor Zyla
Nikon Eclipse Ti2 20X
40X
Laser Lines:
405nm
408nm
561nm
640nm
DAPI 405/455
CFP 405/480
GFP 488/525
mCh 561/605
Cy5 640/705
W1 Yokogawa Spinning Disk (50μm pinholes) Andor Zyla