Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Bacterial and virus strains | ||
| DH5α | ThermoFisher | Cat# 18265017 |
| DH10β | ThermoFisher | Cat# EC0113 |
| T7 Express | New England Biolab | Cat# C2566 |
| E cloni 10G Supreme | Lucigen | Cat# 60107-1 |
| BL21(DE3) | Novagen | Cat# 69450 |
| Chemicals, peptides, and recombinant proteins | ||
| Lipofectamine 2000 | ThermoFisher | Cat# 11668030 |
| Blasticidin | ThermoFisher | Cat# A1113903 |
| Puromycin | ThermoFisher | Cat# A1113803 |
| Doxycycline | ThermoFisher | Cat#J67043-AE |
| Isopropyl β- d-1-thiogalactopyranoside (IPTG) | Gold Bio | Cat# I2481C |
| phenylmethylsulfonyl fluoride (PMSF) | Millipore Sigma | Cat#P7626 |
| Imidazole | MP Biomedicals | Cat# 102033 |
| Tris base | Fisher Chemical | Cat#BP152-5 |
| Glucose | Fisher Chemical | Cat#D16-500 |
| Critical commercial assays | ||
| Pierce™ BCA Protein Assay Kit | ThermoFisher | Cat#23225 |
| Deposited data | ||
| Stitched brightfield images of uninduced and induced cell aggregate for aggregate size analysis | This paper | 10.17632/5j93mffst8.1 |
| Stitched 3-pair co-culture images used for imaging-based interaction analysis | This paper | 10.17632/5j93mffst8.1 |
| Raw OD650 data for HCSRA | This paper | 10.17632/5j93mffst8.1 |
| Images used for analyzing CC-His affinity | This paper | 10.17632/5j93mffst8.1 |
| Experimental models: Cell lines | ||
| K562 | ATCC | CCL-243 |
| HEK293AAV | Agilent | 240073 |
| Hek293T | ATCC | CRL-3216 |
| Experimental models: Organisms/strains | ||
| S. Cerevisiae SynAg MATa | Kavins Lab | N/A |
| S. Cerevisiae SynAg MAT□ | Kavins Lab | N/A |
| Recombinant DNA | ||
| Plasmid: PB_TRE_Z17-PDGFR_EF1a_mCherry | This Paper | Addgene 186303 |
| Plasmid: PB_TRE_Z18-PDGFR_EF1a_eGFP | This Paper | Addgene 186304 |
| Plasmid: PB_TRE_P3-PDGFR_EF1a_iRFP670 | This Paper | Addgene 186305 |
| Plasmid: PB_TRE_AP4-PDGFR_EF1a_eBFP2 | This Paper | Addgene 186306 |
| Plasmid: PB_TRE_P9-PDGFR_EF1a_mPlum | This Paper | Addgene 186307 |
| Plasmid: PB_TRE_AP10-PDGFR_EF1a_mOrange | This Paper | Addgene 186308 |
| Plasmid: PB_TRE_sg30-PDGFR_EF1a_mCherry | This Paper | Addgene 186309 |
| Plasmid: PB_TRE_sg61-PDGFR_EF1a_eGFP | This Paper | Addgene 186310 |
| Plasmid: PB_TRE_sg83-PDGFR_EF1a_iRFP670 | This Paper | Addgene 186311 |
| Plasmid: PB_TRE_sg88-PDGFR_EF1a_eBFP2 | This Paper | Addgene 186312 |
| Plasmid: pQE80_T5_Z17-ehaA_LacI | This Paper | Addgene 186313 |
| Plasmid: pQE80_T5_Z18-ehaA_LacI | This Paper | Addgene 186314 |
| Plasmid: pQE80_T5_P3-ehaA_LacI | This Paper | Addgene 186315 |
| Plasmid: pQE80_T5_AP4-ehaA_LacI | This Paper | Addgene 186316 |
| Plasmid: pQE80_T5_P9-ehaA_LacI | This Paper | Addgene 186317 |
| Plasmid: pQE80_T5_AP10-ehaA_LacI | This Paper | Addgene 186318 |
| Plasmid: pET22b_His-sg30 | This Paper | N/A |
| Plasmid: pET22b_His-sg61 | This Paper | N/A |
| Plasmid: pET22b_His-sg83 | This Paper | N/A |
| Plasmid: pET22b_His-sg88 | This Paper | N/A |
| Plasmid: pCAG-sg30-TEVs-GFP-His | This Paper | N/A |
| Plasmid: pCAG-sg61-TEVs-GFP-His | This Paper | N/A |
| Plasmid: pCAG-sg83-TEVs-GFP-His | This Paper | N/A |
| Plasmid: pCAG-sg88-TEVs-GFP-His | This Paper | N/A |
| Plasmid: PB-sg61-PDGFR-blast | This Paper | N/A |
| Plasmid: PB-sg88-PDGFR-blast | This Paper | N/A |
| Library general plasmid: pQE80X_Z17-autotransporter | This Paper | N/A |
| Library general plasmid: pSYNAGaV3_sgX_SUMO_kan | This Paper | N/A |
| Library general plasmid: pETDEST_sGFP_sgX | This Paper | N/A |
| Software and algorithms | ||
| CellProfiler | Stirling, et al. 2021 | https://cellprofiler.org/ |
| ImageJ | Schneider et al., 2012 | https://imagej.nih.gov/ij/ |
| Software and algorithms | ||
| CellProfiler | Stirling, et al. 2021 | https://cellprofiler.org |
| ImageJ | Schneider et al., 2012 | https://imagej.nih.gov/ij/ |
| Zen Blue | Zeiss | https://www.zeiss.com/microscopy/us/products/microscope-software/zen.html |
| Nikon Elements | Nikon | https://www.microscope.healthcare.nikon.com/products/software/nis-elements |
| A Plasmid Editor (ApE) | M. Wayne Davis | https://jorgensen.biology.utah.edu/wayned/ape/ |
| Agilent Mass Hunter | Agilent | https://www.agilent.com/en/promotions/masshunter-mass-spec |
| MATLAB | Mathworks | https://www.mathworks.com/products/matlab.html |
| Python | Python Software Foundation | https://www.python.org/ |
| BioPython | Cock, et al. 2009 | https://biopython.org/ |
| Sickle | N/A | https://github.com/najoshi/sickle |
| Code for Image-based Frequency Analysis and HCSRA | This paper | 10.5281/zenodo.6323728 |
| ProtParam | ExPasy | https://web.expasy.org/protparam/ |
| Other | ||
| Ultra-Low-Adherence v-bottom plates | S-Bio | Cat# MS-9096VZ |
| Coiled-Coil and helixCAM Amino Acid Sequences | This Paper | S26 |