Figure S2.
PAE plots and pLDDT scores. (A–C) PAE plots (left) and per-residue confidence score (pLDDT) of the three Spindly models discussed in the main text. (A) Spindly1–275. (B) Spindly1–309. (C) Spindly1–440. The pLDDT scores are displayed on the AF2 Multimer predictions of Spindly shown on the right (Blue: high confidence; Red: low confidence). The models are shown, with the same orientation, in Figs. 1 and 2. The PAE matrices refer to models of Spindly dimers, and correspondingly numbering of residues on the left and bottom of the plot is the number of residues in each chain multiplied by 2, and the second chain is plotted directly following the first. The parallel coiled-coils give rise to off-diagonal signals (blue) parallel to the main diagonal. Besides straight models like the one shown, a few predicted models of Spindly1–275 also showed a folded-back conformation. However, there are no additional off-diagonal signals for the Spindly1–275 construct in the PAE plots, suggesting that even if Spindly1–275 explores folded-back conformations, these are not stable. Off-diagonal signals perpendicular to the main diagonal are instead clearly visible in the Spindly1–309 and Spindly1–440 constructs, consistent with a folded back conformation. A predicted folded conformation of Spindly1–440 was also observed with orthologous sequences (unpublished results).