Figure S5.

Additional analyses of the Spindly N-terminal autoinhibitory region. (A–D) Additional analytical SEC interaction assays between the Dynactin PE (brown) and the indicated Spindly constructs. The complex run is always represented with a continuous line, the Spindly construct with a dashed line. (A) Spindly^1–440 (blue); Spindly^1–354 (green). (B) ^mChSpindly^76–605 (blue). (C) ^mChSpindly^Δ26–28 (green). (D) ^mChSpindly^Δ26–32 (blue). PE: 3 µM; Spindly construct: 8 µM. The PE alone control in A is shared with Fig. 3 D, between C and D, and between B and Fig. S6, A and B. mAU, milli absorbance units. Molecular weights are in kD. Source data are available for this figure: SourceData FS5.