Figure S6.
Additional characterization of Spindly binding to PE. (A) SEC separation of GSTSpindly (dotted green line), the PE complex (brown line), and their mixture (continuous green line). (B) SEC separation of GSTSpindlyCC2* (dotted green line), the PE complex (brown line), and their mixture (continuous green line). The PE control is shared between the two shown experiments and with Fig. S3 B. PE: 3 µM, Spindly construct: 8 µM. (C) The AF2 ColabFold model of the BicD2-Spindly chimera shows CC1 is continuous. (D) Spinning-disk confocal fluorescence microscopy-based filamentation assay at 561 nm with the indicated mChRZZSF species (4 µM RZZ, 8 µM SpindlyF) at 20°C in absence of MPS1 kinase. mAU, milli absorbance units. Molecular weights are in kD. Scale bar: 5 µm. Source data are available for this figure: SourceData FS6.