Skip to main content
. 2000 Dec;182(24):6933–6939. doi: 10.1128/jb.182.24.6933-6939.2000

FIG. 2.

FIG. 2

Plasmid constructions used in this study (A) and EcoRV restriction map of the ppt1 and the ppt1::ura4 regions (B). Asterisks indicate the sites of TA ligation with the T-tailed vector pT7Blue-T. pREP1-PPT1 and pREP1-COQ2 contain the entire lengths of the ppt1 and COQ2 genes, respectively, and are under the control of the strong nmt1 promoter. pGFP-TP45 and pGFP-TP68 contain putative mitochondrial transit peptides (TP) of Ppt1. Thin arrows indicate the direction and the length of open reading frames. Abbreviations for restriction enzymes: B, BamHI; E, EcoRV; Sa, SalI; Sc, SacI; Sn, SnaBI; Sm, SmaI.