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Detection of UQ-10. Ubiquinone was extracted from the wild-type SP826, NU609 (Δppt1::ura4), NU609 harboring plasmid pREP1-PPT1, and NU609 harboring plasmid pREP1-COQ2. Ubiquinone was first separated by thin-layer chromatography and then further analyzed by high-pressure liquid chromatography.
