Figure 2.

Yun represses GSC differentiation

(A) Bam (red) in control germarium (GSCs, white ovals).

(B and C) Bam (red) in nos > yun^shmiR germarium (GSCs, white ovals).

(D) Quantification of Bam fluorescence in control and nos > yun^shmiR GSCs. Mean ± SEM is shown. n = 15. ^∗∗∗∗p < 0.0001.

(E) Bam (red) in nos > stop > GFP germarium (GSCs, white ovals).

(F and G) Bam (red) in nos > stop > yun^shmiR germarium (yellow ovals and white arrowheads).

(H) Quantification of Bam fluorescence in control and nos > stop > yun^shmiR GSCs. Mean ± SEM is shown. n = 12. ^∗∗∗∗p < 0.0001.

(I) Bam (red) in marked control clones (oval) induced in adult flies.

(J and K) Bam (red) in marked yun mutant clones (yellow ovals).

(L) Quantification of Bam fluorescence in control and yun mutant GSCs. Mean ± SEM is shown. n ≥ 12. ^∗∗∗∗p < 0.0001.

(M) pMAD (red) and Bam-GFP (green) in control germarium (GSCs, white ovals). Bam-GFP channel is showed separately in black and white.

(N and O) pMAD (red) and Bam-GFP (green) in nos > yun^shmiR germarium (GSCs, yellow ovals).

(P) Quantification of Bam-GFP fluorescence in control and nos > yun^shmiR GSCs. Mean ± SEM is shown. n = 12. ^∗∗∗∗p < 0.0001.

(Q) Quantification of pMAD fluorescence in control and nos > yun^shmiR GSCs. Mean ± SEM is shown. n = 12. ^∗∗∗∗p < 0.0001.

(R) pMAD (red, white arrowheads) in marked nos > stop GSCs (white ovals).

(S) pMAD (red, arrowheads) in marked nos > stop > yun^shmiR GSCs (yellow oval). pMAD channel is shown separately.

(T) Quantification of pMAD fluorescence in control and nos > stop > yun^shmiR GSCs. Mean ± SEM is shown. n = 12. ^∗∗∗∗p < 0.0001.

(U) pMAD (red, white arrowhead) in marked control GSCs (white ovals).

(V and W) pMAD (red, white arrowheads) in marked yun mutant clones (yellow ovals).

(X) Quantification of pMAD fluorescence in control and yun mutant GSCs. Mean ± SEM is shown. n = 10. ^∗∗∗∗p < 0.0001. Scale bars: 10 μm.