Figure 4.

Yun functions through Tkv to maintain GSC

(A) GSCs (by pMAD in red, white arrowheads) in control germarium.

(B) nos > tkv^QD germaria contain numerous GSC-like cells (white arrowheads).

(C) No GSCs (by pMAD in red, white arrowheads) can be detected in nos > yun^shmiR germarium.

(D) nos > tkv^QD, yun^shmiR germaria contain numerous GSC-like cells (white arrowheads).

(E) Quantification of pMAD fluorescence in GSCs with indicated genotypes. Mean ± SEM is shown. n = 12. ^∗∗∗∗p < 0.0001.

(F) Vasa (red) and 3A9 (green) in control germarium (white arrowheads).

(G) Vasa (red) and 3A9 (green) in nos > tkv^QD germarium. All germaria contain numerous GSC-like cells (white arrowheads).

(H) Vasa (red) and 3A9 (green) in nos > yun^shmiR germarium that contains developing cysts (yellow oval).

(I) Vasa (red) and 3A9 (green) in nos > tkv^QD, yun^shmiR germarium. All germaria contain numerous GSC-like cells (white arrowheads).

(J) Bam (red, white arrowhead) in control germarium.

(K) Bam (red) is barely detected in all nos > tkv^QD germaria.

(L) Bam (red, white arrowheads) is expressed in putative GSCs (yellow ovals) in the niche of nos > yun^shmiR germarium.

(M) Bam (red) is barely detected in all nos > tkv^QD, yun^shmiR germaria.

(N) Quantification of Bam fluorescence in GSCs with indicated genotypes. Mean ± SEM is shown. n = 15. ^∗∗∗∗p < 0.0001. Scale bars: 10 μm.