Figure 5.

CXCL10 KO SC-islet grafts evade alloimmune attack in humanized mice

(A) WT or C10G SC-islets were transplanted into MHC^null NSG mice (n = 10 from each line). n = 6–7 mice from each group injected with human PBMCs (n = 2 human donors), while the remainder served as control (n = 3 per group).

(B) Graft failure at week 11 after PBMC injections, as measured by human insulin in fasted mice plasma, 30 min after glucose injection to fasted mice. Data presented as fold increase from t = 0 before PBMC injections.

(C) Flow cytometry of SC-α (glucagon+/C-peptide−) and SC-β (glucagon−/C-peptide+) in extracted grafts at week 18 post PBMC injection. n = 3–4 mice per group.

(D) Flow cytometry of human T cells in hPi-mouse graft infiltrating at week 18 post PBMC injection. n = 3–5 mice per group. Error bars are mean ± SD. ns, not significant; ^∗p < 0.05; ^∗∗p < 0.01, unpaired two-tailed t test.