Figure 2.

Characterization of MOSs

(A) Representative bright-field images of human colon bulk organoids and MOSs in expansion (WENR) and differentiation (EN) medium accompanied by H&E images of the same cultures (scale bar: 100 μm).

(B) Representative staining of Ki67 and MUC2 for human colon bulk organoids and MOSs cultured in WENR and EN medium.

(C) Bar graphs showing the quantification of the MUC2 and Ki67 staining (bars show the mean ± SEM of n = 10 different images).

(D) Bar graphs representing gene expression of selected markers for MOS and bulk cultures in WENR versus EN medium (bars show the mean ± SEM of n = 3).

(E) Representative confocal image of Ezrin reporter MOS (scale bar: 500 μm).

(F) Representative images of the Ezrin reporter MOS under normal culture condition (top) or after BME removal (bottom) (scale bar: 200 μm).