Skip to main content
. 2022 Aug 18;17(9):1959–1975. doi: 10.1016/j.stemcr.2022.07.016

Figure 3.

Figure 3

MOSs enable uniform nutrient accessibility

(A) Representative images of CRC#1, CRC#2, CRC#3, and a distal lung line growing in bulk organoid cultures or as MOSs (scale bar: 200 μm).

(B) Duodenum organoids in BME dome in presence of 17.5 mM glucose (scale bar: 500 μm).

(C) Duodenum organoids cultured in BME domes were stained with CAM (scale bar: 2,000 μm).

(D) Duodenum organoids cultured in MOS in presence of 17.5 mM glucose (scale bar: 500 μm).

(E) Representative images of duodenum MOSs after 0 or 7 days of treatment with different glucose (Glc) or fructose (Frc) concentrations (scale bar: 2,000 μm). MOSs were stained with CAM.

(F and G) Image-based quantification of the green area visualized for each condition as mean values ± SD (n = 6 from two biological replicates) after 7 days (F) or over time (G).