Fig. 2.

Integrin α5 and Fn1 co-localize in beaded adhesions. Wild-type MEFs were cultured for 16 h on glass coverslips without coating, fixed and stained with the Abcam monoclonal Fn1 antibody (cyan) and anti-integrin α5 (Itga5) antibody (magenta). Cells were imaged at the critical angle of incidence using a 100× oil objective, with NA 1.49. (A–A2) Representative images of the cell periphery. Arrows in A–A2 point to examples of non-fibrillar Fn1 adhesions. (B–B2) Representative images of the medial portion of a cell containing beaded fibrillar adhesions (arrows). Magnifications in all panels are the same. Images are representative of three experiments. Scale bar: 2 μm