Fig. 4.
Nanodomain architecture of Fn1 fibrils detected with four different antibodies. Fn1mEGFP/mEGFP MEFs were plated on glass coverslips without coating, cultured overnight and stained with the following antibodies: (A) R457 (1:200 dilution); (B) R184 (1:100 dilution); (C) Abcam monoclonal antibody (1:200 dilution); and (D) anti-GFP antibody (1:100 dilution). Boxed regions in A–D are magnified to show fibrils in A1–D1, and bracketed regions in A1–D1 are magnified in A2-D2. Fourier ring correlation (FRC) was used to determine image resolution. The vertical bar in D shows color coding according to localization density for all panels in this figure. Cells were imaged using SMLM imaging protocol I. Arrowheads point to examples of sparse Fn1 localizations between nanodomains. Images are representative of three independent experiments. Scale bars: 10 μm (A–D); 1 μm (A1–D1); 100 nm (A2–D2).