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. 2021 Nov 26;40:167–178. doi: 10.1016/j.jare.2021.11.015

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© 2022 The Authors. Published by Elsevier B.V. on behalf of Cairo University.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 6 — Agr inactivation resulted in comparable vancomycin resistance and bacterial virulence. (A) E-test of vancomycin susceptibilities in XN108/Δagr, XN108-R/Δagr, USA300/Δagr, K-USA300/Δagr, Newman/Δagr, and K-Newman/Δagr. RT-qPCR detection of the expression levels of virulence factors in (B) XN108/Δagr and XN108-R/Δagr, (C) USA300/Δagr and K-USA300/Δagr, and (D) Newman/Δagr and K-Newman/Δagr. Statistical significance was calculated by 2way ANOVA. * P < 0.05, *** P < 0.001, and ns indicates no significance. Cross streak assay for (E) XN108 and its derivatives, (F) USA300 and its derivatives, and (G) Newman and its derivatives. S. aureus RN4220 was used to represent Hlb production. The production of other hemolysins was indicated.