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. 2022 Aug 22;149(16):dev200499. doi: 10.1242/dev.200499

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© 2022. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 6. — TEM analysis. (A-L) Ultrastructural analysis using TEM on testes sections of WT, Pfn4^+/− and Pfn4^−/− mice (n=3 biological replicates/genotype). (A-C) Golgi phase spermatozoa of WT (A), Pfn4^+/− (B) Pfn4^−/− (C) mice (arrow indicates deformed developing acrosome). Red arrow indicates the acroplaxome, red dashed rectangles indicate the marginal ring. (D-F) Cap phase spermatozoa of WT (D), Pfn4^+/− (E) and Pfn4^−/− (F) mice (arrow indicates abnormal cap-like structures). (G-I) Acrosomal phase spermatids of WT (G), Pfn4^+/− (H) and Pfn4^−/− (I) mice. (J-L) Maturation phase spermatids of WT (J), Pfn4^+/− (K) and Pfn4^−/− (L) mice. (M-O) Ultrastructural analysis of cauda epididymal sperms of WT (M), Pfn4^+/− (N) and Pfn4^−/− (O) mice. Scale bars: 2 μm.