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. 2022 Aug 22;149(16):dev200499. doi: 10.1242/dev.200499

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© 2022. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 9. — Acrosomal reaction and in vitro fertilization. Calcium ionophore-induced acrosomal reaction in WT, Pfn4^+/− and Pfn4^−/− sperm cells (n=3/genotype). (A) Coomassie-stained sperm cells of WT, Pfn4^+/− and Pfn4^−/−. Black arrows indicate that a successful acrosomal reaction took place. Black arrowhead indicates a crescent-shaped acrosome on a sperm head, indicating that the acrosomal reaction did not take place. Scale bars: 20 μm. (B) Percentage of acrosomal-reacted sperm WT, Pfn4^+/− and Pfn4^−/− (n=3 biological replicates/genotype). ***P<0.0005, one-tailed, paired Student's t-test. P-value relates to acrosomal-reacted WT and Pfn4^+/− sperm compared with Pfn4^−/− sperm. (C) Percentage of oocytes developing into blastocysts after fertilization of oocytes with WT and Pfn4^−/− sperm and zona-free oocytes with Pfn4^−/− sperm (n=1/genotype).