The following antibodies were used for Western blotting.
Antibody | Dilution | Source | Reference |
---|---|---|---|
Anti-hexokinase rabbit IgG | 1:8,000 | US Biological | H2035 |
Anti-FLAG mouse monoclonal IgG1, clone M2 | 1:2,000 | Sigma-Aldrich (Merck) | F3165 |
Amersham ECL anti-mouse IgG, HRP-linked whole antibody (from sheep) | 1:10,000 | GE Life Sciences | NA931V5 |
Amersham ECL anti-rabbit IgG, HRP-linked whole antibody (from donkey) | 1:10,000 | GE Life Sciences | NA934V |