Figure 1.

Development of RNA-Pol-II-mediated transcription of pegRNA editing system

(A) Schemes for the different production modes of pegRNAs. p2PE3-2 utilized CAG promoter to drive expression of mCherry transcript harboring HSV-1 intron containing gRNA cassette connected by Csy4 recognition sites. E, exon. (B) Comparison of substitution edits with PE3, ePE3, p2PE3-1, and p2PE3-2 at 10 endogenous target sites in HEK293T cells is shown. (C and D) Statistical analysis of the editing frequency (C) and indels (D) induced by four editing systems in (B) is shown. (E) Comparison of editing and indel frequencies with ePE3 and p2PE3 targeting six individual sites for three deletions and three insertions in HEK293T cells is shown. (F) Statistical analysis of intended editing and indel frequencies from (E) is shown. Data were represented as the mean ± SEM (n = 3 from independent experiments). Two-tailed Student’s t tests were performed.