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. 2022 Jul 6;30(9):2923–2932. doi: 10.1016/j.ymthe.2022.07.002

Figure 2.

Figure 2

p2PE3 outperforms ePE3 with pegRNA containing poly-T

(A) Efficiency of ePE3 and p2PE3-mediated insertions of poly(A) at four endogenous target sites in HEK293T cells. (B) Schematic of a pegRNA containing poly(T) to target genome site is shown. PBS, primer binding site; RTT, reverse transcription template. (C) The proportion of pegRNA containing poly(T) to target known human pathogenic genetic variants is shown. (D) Efficiency of ePE3 and p2PE3 with pegRNA containing poly(T) at six human pathogenic sites in HEK293T cells is shown. Data and error bars in (A) and (D) indicated the mean ± SEM of three independent experiments.