Figure 3.

p2PE3 has an advantage in combinatorial genetic editing

(A) Diagram of the dual fluorescence reporter system for comparing combinatorial genetic editing. The prime editors can repair the 1-bp frameshift insertion and restore the fluorescence of mCherry and EGFP. (B) Statistical analysis of normalized fluorescence ratio is shown, setting the individual gRNA cassette restoring mCherry or EGFP to 1. (C) Schematic of three multiplexed arrays with pegRNA-nicking sgRNA cassettes targeting MKL2, MYH7, and NKX2-5 is shown. (D) Efficiency of ePE3 and p2PE3 to model childhood-onset cardiomyopathy mutations using individual gRNA cassettes or multiplexed arrays is shown. (E) Statistical analysis of normalized efficiency of individual gRNA cassettes or gRNA cassettes in the different position of arrays in (D) is shown, setting the individual gRNA cassettes of ePE3 to 1. Data were represented as the mean ± SEM (n = 3 from independent experiments). Two-tailed Student’s t tests were performed.