Figure 4.

Controllable prime editing achieved by p2PE3 in hESCs

(A) Comparison of the different strategies to improve editing efficiency of base substitution and small targeted deletion in hESCs. Co-expression of p2PE3 with dominant negative MLH1 (p2PE5), SV40 large T antigen (p2PE3+SV40LT), or both (p2PE5+SV40LT) was tested. (B) Statistical analysis of the editing frequency in (A) is shown. Two-tailed Student’s t tests were performed. (C) Editing and indel frequencies of inducible p2PE3 system combining with PE5 and SV40LT strategies are shown, comparing with or without doxycycline (Dox). Data were represented as the mean ± SEM (n = 3 from independent experiments).