Table 1.
Spacer and 3ʹ extension sequences of pegRNAs and spacer sequences of ngRNAs
| Spacer sequence | 3ʹ extension sequence | PBS length (nt) | RTT length (nt) | ||
|---|---|---|---|---|---|
| pegRNA | HEK3 +1 CTT insertion | 5ʹ-GGCCCAGACTGAGCACGTGA-3ʹ | 5ʹ-AGACTTCTCCACA GGAGTCAGGTGCAC-3ʹ |
13 | 10 |
| HBB +3 A to T | 5ʹ-GCATGGTGCACCTGACTCCTG-3ʹ | 5ʹ-AGACTTCTCCACAG GAGTCAGGTGCAC-3ʹ |
13 | 14 | |
| RNF2 +5 G to T | 5ʹ-GTCATCTTAGTCATTACCTG-3ʹ | 5ʹ-AACGAACACATCA GGTAATGACTAAGATG-3ʹ |
15 | 14 | |
| PCSK9 +1 A insertion | 5ʹ-CCCATACCTTGGAGCAACGG-3ʹ | 5ʹ-ACCTTCCGCCGaT TGCTCCAAGGT-3ʹ |
12 | 12 | |
| ngRNA | HEK3 +1 CTT insertion | 5ʹ-GTCAACCAGTATCCCGGTGC-3ʹ | |||
| HBB +3 A to T | 5ʹ-CCTTGATACCAACCTGCCCA-3ʹ | ||||
| RNF2 +5 G to T | 5ʹ-TCAACCATTAAGCAAAACAT-3ʹ | ||||
| PCSK9 +1 A insertion (ngRNA1) | 5ʹ-ACCTTCCGCCGaTTGCTCCA-3ʹ | ||||
| PCSK9 +1 A insertion (ngRNA2) | 5ʹ-CCGCCGaTTGCTCCAAGGTA-3ʹ |