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. 2022 Jun 22;30(9):3034–3051. doi: 10.1016/j.ymthe.2022.06.012

Figure 2.

Figure 2

The structure of PEG lipid in the LNP compositions reduces the potency of siRNA-mediated knockdown of GNAS in vitro

(A–C) MSC-like cells were seeded in multi-well plates and siGNAS or siCtrl encapsulated in LNPs containing PEG-DMG or PEG-DSG were administered in triplicate wells at indicated doses. Bar graphs represent expression of GNAS mRNA relative to HPRT determined 4 days after incubation with (A) PEG-DMG and (B) PEG-DSG LNPs as determined by qRT-PCR. The GNAS mRNA was normalized to the housekeeping genes HPRT and TBP. GNAS mRNA values following LNP-siCtrl treatments are considered 100%. Each data point represents arithmetic mean ± SD of three technical replicates. Data from one representative experiment of three independent experiments are shown. Bar graphs in (C) represent expression of TBP mRNA relative to HPRT. TBP mRNA following siCtrl is considered to be 100% (∗p < 0.05).