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. 2022 Jun 22;30(9):3034–3051. doi: 10.1016/j.ymthe.2022.06.012

Figure 8.

Figure 8

Silencing of GNAS by LNP-siRNA in C57Bl6 wild-type mice leads to osteogenic differentiation of MSCs in vivo

Osteogenic marker transcripts of PDGFRα+/Sca-1+ MSCs were determined in pooled bones (femur and tibiae) 4 and 8 days following treatment with LNP-siGNAS or LNP-siCtrl (PEG-DSG), using ddPCR. (A) Dot plots (left) display PDGFRα+/Sca-1+ MSCs following administration of siCtrl and dot plots (right) display PDGFRα+/Sca-1+ MSCs following administration of siGNAS. Bar graphs (middle) depict mRNA GNAS transcripts of PDGFRα+/Sca-1+ cell populations relative to siCtrl at indicated time points. mRNA GNAS transcripts following siCtrl treatment were considered to be 100%. (B and C) Bar graphs depict GNAS mRNA transcripts of osteogenic markers relative to siCtrl of PDGFRα+/Sca-1+ MSC populations at (B) 4 days and (C) 8 days following systemic administration of LNP-siRNA. mRNA GNAS transcripts following siCtrl treatment were considered to be 100%. Each data point represents arithmetic mean ± SD of two mice. Data from one representative experiment of two independent experiments are shown (∗p < 0.05).