FIG. 2.

Deletion analysis of the 10.5-kb EcoRI fragment and lig gene organization. The OMA hydratase activity of cells containing each subclone is presented on the right. Arrows indicate the direction of transcription from the lac promoters. The ligI, ligJ, ligA, ligB, and ligC genes are indicated by filled arrows; a partly filled arrow represents the part of the ORF of the lignostilbene α, β-dioxygenase homolog (lsdA). Each plasmid was introduced in E. coli JM109. E, EcoRI; P, PstI; Sl, SalI; Sm, SmaI; X, XhoI; Xb, XbaI.