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Purification of OMA hydratase from E. coli BL21(DE3) harboring pETJ
| Fraction | Total protein (mg) | Total activity (U) | Sp act (U/mg) | Yield (%) | Fold purification |
|---|---|---|---|---|---|
| Crude extract | 72.0 | 3,100 | 43.1 | 100 | 1.0 |
| PI | 8.21 | 1,380 | 168 | 45 | 3.9 |
| HQ | 3.00 | 921 | 307 | 30 | 7.1 |
| PE | 1.10 | 613 | 557 | 20 | 13 |
