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. Author manuscript; available in PMC: 2022 Sep 17.
Published in final edited form as: Biochemistry. 2022 Jul 27;61(16):1669–1682. doi: 10.1021/acs.biochem.2c00224

Figure 2.

Figure 2.

EMSA analysis of FKH binding to the insulin response element (IRE). Representative blots of FKH wt, FKH L183P and FKH L183R serial dilution from 1 μM (1000 nM) to 5 nM in complex with 1 nM dsIRE. Each blot is labeled with KD estimates in nM.