Table 1.
Melting temperatures and enthalpies of unfolding measured by TSA and DSC experiments.
| TSA | DSCc | ||||||
|---|---|---|---|---|---|---|---|
| FKH construct | Tm (°C) | ΔTm | Tm (°C) | ΔTm | ΔHcal (kcal/mol) | ΔHvH (kcal/mol) | ΔΔHvH-cal (kcal/mol) |
| WT | 50.5±0.8 | - | 57.8 ± 0.3 | - | 47 ± 1 | 54 ± 1 | 7 |
| S152R | 51.1±0.1 | 0.6 | 57.0 ± 1.0 | −0.8 | 51 ± 1 | 58 ± 1 | 7 |
| S153R | 51.8±0.1 | 1.2 | 58.6 ± 1.0 | 0.8 | 52 ± 1 | 60 ± 1 | 8 |
| A166G | 46.4±0.5 | −4.2 | 55.1 ± 1.0 | −2.7 | 44 ± 1 | 54 ± 1 | 10 |
| A166V | 56.3±0.9 | 5.8 | 61.9 ± 1.0 | 4.2 | 47 ± 1 | 68 ± 1 | 21 |
| K171E | 52.5±0.8 | 2.0 | – | - | - | - | |
| T182Ma | 46.4±0.1 | −4.1 | 51.5 ± 1.0 | −6.3 | 40 ± 1 | 62 ± 1 | 22 |
| L183Rb | 33.6±1.1 | −16.9 | 41.7 ± 1.0 | −16.1 | 50 ± 1 | 42 ± 1 | −8 |
| L183P | 40.0±0.6 | −10.5 | 48.2 ± 1.0 | −9.6 | 31 ± 1 | 53 ± 1 | 22 |
| S205N | 52.4±1.1 | 1.9 | - | - | - | - | |
| S205T | 52.7±1.7 | 2.1 | 58.3 ± 1.0 | 0.5 | 58 ± 1 | 59 ± 1 | 1 |
a
Mutation in OCI-Ly8 cell line.
b
Mutation in WSU-NHL cell line.
c
Wild-type measurements were performed in triplicate (± 0.3 °C), while single measurements were made for mutants (± 1.0 °C)