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SDS-PAGE of desalted 20 to 50% saturated ammonium sulfate extracts of isopropyl-β-d-thiogalactopyranoside-induced E. coli BL21(DE3) that was transformed with the blank cloning vector (pET16b) (lane 2) or with a vector containing Rv1170 (pYA1170b) (lane 3). Bio-Rad Broad Range molecular mass standards (lanes 1 and 5) and purified M. smegmatis mycothiol S-conjugate amidase (lane 4) are also shown.
