Figure 3.

Histological evaluation of the primary tumors and distant organs for metastasis

Paraffin embedded tissue sections were stained for H&E and microscopic examination of the tumors formed by WT and KO MDA-MB-231 cells. Primary tumors as well as tumor cells in the lymph nodes, lung, and liver tissues were shown. Dashed line circles show metastasized tumors in lymph node, lung and liver. Images are representatives from 5 mice received WT cells and 5 mice received KO cells. T: Tumor. Scale bar = 50 μm, magnification is 40× except for lymph nodes which is 20×. Bottom panels show the quantification of the cytokeratin 18 positive lesions in the liver (left) and the lung (right). Data are represented as mean ± SD.