Antibodies |
PE anti-mouse/human CD11b (clone M1/70) |
Biolegend |
Cat. # 101208 |
Percp cy5.5 anti-mouse CD3 (clone 17A2) |
Biolegend |
Cat. # 100218 |
PE anti-mouse CD4 (Clone GK1.5) |
Biolegend |
Cat. # 100408 |
PEcy7 anti-mouse CD11c (clone N418) |
Biolegend |
Cat. # 117318 |
APC anti-mouse MHCII (clone AF-120.1) |
Biolegend |
Cat. # 116418 |
Pacific blue anti-mouse Ly6G (clone 1A8) |
Biolegend |
Cat. # 127612 |
APC/Cyanine7 anti-mouse CD19 (Clone 6D5) |
Biolegend |
Cat. # 115530 |
FITC anti-mouse CD86 (clone PO3) |
Biolegend |
Cat. # 105110 |
FITC anti-mouse IL-2 (clone JES6-5H4) |
Biolegend |
Cat. # 503806 |
LPS from E. coli, Serotype O55:B5 (TLRGRADE) |
Enzo Life Sciences |
Cat. # ALX-581-013 |
Diphtheria Toxin from Corynebacterium diphtheriae
|
Sigma-Aldrich |
Cat. # D0564 |
|
Chemicals, peptides, and recombinant proteins |
|
FK506 monohydrate |
Sigma-Aldrich |
Cat. # F4679 |
Thapsigargin |
Sigma-Aldrich |
Cat. # T9033 |
Poly(isobutylene)-alt-maleic anhydride |
Merk Life Science S.r.l. |
Cat# 531278 |
Dodecylamine |
Merk Life Science S.r.l |
Cat# 44172 |
2,2’-(Ethylenedioxy)bis(ethylamine) (EDBE) |
Merk Life Science S.r.l. |
Cat# 385506 |
N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC) |
Merk Life Science S.r.l. |
Cat#03450 |
(sulfosuccinimidyl 6-[3′-(2-pyridyldithio)propionamido]hexanoate) (sulfo-LC-SPDP) |
Covachem, LLC. |
Cat# 13412 |
PEG-SH 550 Da |
Biochempeg scientific inc. |
Cat#20001-550 |
VIVIT peptide |
Laura Marongiu et al. 2021, https://doi.org/10.1126/scisignal.aaz2120
|
N/A |
H-Ferritin (HFn) |
Tullio et al., (2021)https://doi.org/10.1021/acsabm.1c00724
|
N/A |
|
Critical commercial assays |
|
IFN gamma Mouse ELISA Kit |
Invitrogen |
Cat #BMS606-2 |
CD4+ T Cell Isolation Kit, mouse |
Miltenyi Biotec |
Cat #130-104-454 |
RNeasy Mini Kit |
Qiagen |
Cat #74104 |
High-Capacity cDNA Reverse Transcription Kits |
Applied Biosystems |
Cat # 4368814 |
|
Experimental models: Cell lines |
|
A20 |
ATCC |
TIB-208 |
|
Experimental models: Organisms/strains |
|
Mouse: C57BL/6 |
Charles River |
C57BL/6NCrl |
Mouse: B6.SJL-Ptprca Pepcb/BoyJ or B6 CD45.1 |
Jackson laboratory |
002014 |
Mouse: Tg(KRT5-TFRC/OVA)1Ita or K5.mOVA |
Jackson laboratory |
No more available |
|
Oligonucleotides |
|
Taqman probe: Ifng |
Thermo Fisher scientific |
Mm01168134_m1; |
Taqman probe: Gapdh |
Thermo Fisher scientific |
Mm99999915_g1 |