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. 2022 Aug 30;25(10):105042. doi: 10.1016/j.isci.2022.105042
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
PE anti-mouse/human CD11b (clone M1/70) Biolegend Cat. # 101208
Percp cy5.5 anti-mouse CD3 (clone 17A2) Biolegend Cat. # 100218
PE anti-mouse CD4 (Clone GK1.5) Biolegend Cat. # 100408
PEcy7 anti-mouse CD11c (clone N418) Biolegend Cat. # 117318
APC anti-mouse MHCII (clone AF-120.1) Biolegend Cat. # 116418
Pacific blue anti-mouse Ly6G (clone 1A8) Biolegend Cat. # 127612
APC/Cyanine7 anti-mouse CD19 (Clone 6D5) Biolegend Cat. # 115530
FITC anti-mouse CD86 (clone PO3) Biolegend Cat. # 105110
FITC anti-mouse IL-2 (clone JES6-5H4) Biolegend Cat. # 503806
LPS from E. coli, Serotype O55:B5 (TLRGRADE) Enzo Life Sciences Cat. # ALX-581-013
Diphtheria Toxin from Corynebacterium diphtheriae Sigma-Aldrich Cat. # D0564

Chemicals, peptides, and recombinant proteins

FK506 monohydrate Sigma-Aldrich Cat. # F4679
Thapsigargin Sigma-Aldrich Cat. # T9033
Poly(isobutylene)-alt-maleic anhydride Merk Life Science S.r.l. Cat# 531278
Dodecylamine Merk Life Science S.r.l Cat# 44172
2,2’-(Ethylenedioxy)bis(ethylamine) (EDBE) Merk Life Science S.r.l. Cat# 385506
N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC) Merk Life Science S.r.l. Cat#03450
(sulfosuccinimidyl 6-[3′-(2-pyridyldithio)propionamido]hexanoate) (sulfo-LC-SPDP) Covachem, LLC. Cat# 13412
PEG-SH 550 Da Biochempeg scientific inc. Cat#20001-550
VIVIT peptide Laura Marongiu et al. 2021, https://doi.org/10.1126/scisignal.aaz2120 N/A
H-Ferritin (HFn) Tullio et al., (2021)https://doi.org/10.1021/acsabm.1c00724 N/A

Critical commercial assays

IFN gamma Mouse ELISA Kit Invitrogen Cat #BMS606-2
CD4+ T Cell Isolation Kit, mouse Miltenyi Biotec Cat #130-104-454
RNeasy Mini Kit Qiagen Cat #74104
High-Capacity cDNA Reverse Transcription Kits Applied Biosystems Cat # 4368814

Experimental models: Cell lines

A20 ATCC TIB-208

Experimental models: Organisms/strains

Mouse: C57BL/6 Charles River C57BL/6NCrl
Mouse: B6.SJL-Ptprca Pepcb/BoyJ or B6 CD45.1 Jackson laboratory 002014
Mouse: Tg(KRT5-TFRC/OVA)1Ita or K5.mOVA Jackson laboratory No more available

Oligonucleotides

Taqman probe: Ifng Thermo Fisher scientific Mm01168134_m1;
Taqman probe: Gapdh Thermo Fisher scientific Mm99999915_g1