Fig. 7.

TD139 dampens microglia reactivity in light-exposed mice. Representative images of Iba-1⁺ cells in the subretinal space of retinal flat mounts from naïve (A) and light-exposed mice treated with vehicle or TD139 (B). Scale bar: 50 µm. C Quantification of Iba-1⁺ area in the subretinal space. n = 15 retinas from individual mice. D Number of Iba-1⁺ cells in the subretinal space. n = 15 retinas from individual mice. E Relative mRNA expression of pro-inflammatory cytokines in retinas of naïve and light-exposed mice treated with vehicle or TD139 normalized to the reference gene Atp5b. n = 6 retinas from individual mice. Representative images of Iba-1⁺ cells in RPE/choroids from naïve (F) and light-exposed mice treated with vehicle or TD139 (G). Scale bar: 50 µm. H Quantification of Iba-1⁺ area RPE/choroidal flat mounts. n = 15 RPE/choroids from individual mice. I Number of Iba-1⁺ cells in the RPE/choroid. n = 15 RPE/choroids from individual mice. J Relative mRNA expression of pro-inflammatory cytokines in RPE/choroids of naïve and light-exposed mice treated with vehicle or TD139 normalized to the reference gene Atp5b. n = 6 RPE/choroids from individual mice. Data show mean ± SEM. *P < 0.05; **P < 0.01 and ***P ≤ 0.001 by ordinary one-way ANOVA followed by Tukey’s multiple comparisons