Table 1.
Summary of early human placenta transcriptome studies
| First author | Year | DOI | Topic | Method | Bulk tissue/cell | Sampling location | Gestational age of sample | Sex reported? (Y/N) | Sex-based analysis? (Y/N) |
|---|---|---|---|---|---|---|---|---|---|
| Tsui | 2004 | https://doi.org/10.1136/jmg.2003.016881 | Identify placental-specific transcripts in maternal blood | Microarray | Bulk tissue | Chorionic villi | 9–12 and 38–40 wks | N | N |
| Mikheev | 2008 | https://doi.org/10.1177/1933719108322425 | Profile placenta across gestation | Microarray | Bulk tissue | Chorionic villi | 6–8 and 15–16 wks | N | N |
| Founds | 2008 | https://doi.org/10.1016/j.placenta.2008.09.015 | Preeclampsia | Microarray | Bulk tissue | Chorionic villi | 10–12 wks | N | N |
| Rull | 2010 | https://doi.org/10.1016/j.placenta.2012.11.032 | Recurrent miscarriage | Microarray | Bulk tissue | Placenta biopsy (chorionic villi + basal plate) | 1st trimester | N | N |
| Sitras | 2012 | https://doi.org/10.1371/journal.pone.0033294 | Placental development across gestation | Microarray | Bulk tissue | Chorionic villi | 9–12 wks | N | N |
| Pantham | 2012 | https://doi.org/10.1016/j.jri.2012.03.487 | Antiphospholipid antibodies | Microarray | Primary explants | Placental tissue explants | 8–8.5 wks | N | N |
| Ghaffari-Tabrizi-Wizsy | 2014 | https://doi.org/10.1159/000381766 | 1st trimester trophoblast vs. 3rd trimester endothelial degradome | Microarray | Primary cell cultures | Chorionic villi (1st trimester), chorionic plate (3rd trimester) | 7–10 wks | N | N |
| Roost | 2015 | https://doi.org/10.1016/j.stemcr.2015.05.002 | Development from early to mid- pregnancy | RNA-seq | Bulk tissue | Amnion, chorion, chorionic villi, decidua, umbilical cord | 8.2–9.6, 16–18, and 21–22 wks | Y | N |
| James | 2015 | https://doi.org/10.1530/REP-14-0646 | Characterize trophoblast subpopulations in early pregnancy | Microarray | Sorted cell (flow cytometry) | Chorionic villi | 5.6–12.5 wks | N | N |
| Leslie | 2015 | https://doi.org/10.1016/j.ajpath.2015.06.020 | Preeclampsia | Microarray | Bulk tissue | Chorionic villi | 9–14 wks | N | N |
| Lassance | 2015 | https://doi.org/10.1016/j.ajog.2015.02.026 | Obesity/Insulin | Microarray | Primary cell culture | Primary cytotrophoblast culture | 7–12 wks | N | N |
| Tian | 2016 | https://doi.org/10.1002/path.4694 | Recurrent miscarriage | Microarray | Primary cell culture | Chorionic villi | 6–12 wks | N | N |
| Söber | 2016 | https://doi.org/10.1038/srep38439 | Recurrent miscarriage | RNA-seq | Bulk tissue | Chorionic villi | 6–12 wks | Y | N |
| Lim | 2017 | https://doi.org/10.1371/journal.pone.0181155 | Placental development across gestation (w/ linked methylation data) | RNA-seq | Bulk tissue | Chorionic villi | 1st and early 2nd trimester | N | N |
| Lim | 2017 | https://doi.org/10.1186/s12864-017-3993-y | Chromosomal abnormalities | Microarray | Bulk tissue | Chorionic villi | 11–13 wks | N | N |
| Weisblum | 2017 | https://doi.org/10.1128/JVI.01905-16 | Zika virus infection | RNA-seq | Organoid cultures | Primary decidual and chorionic villus organoid cultures | Early- and mid-gestation | N | N |
| Soncin | 2018 | https://doi.org/10.1242/dev.156273 | Profile placenta across gestation | Microarray | Bulk tissue | Chorionic villi | 4–39 weeks | N | N |
| Liu | 2018 | https://doi.org/10.1038/s41422-018-0066-y | Characterize placental cell subpopulations | RNA-seq | Single cell w/ pre-sorting (magnetic bead) | Chorionic villi (1st trimester), basal plate (2nd trimester) | 8 and 24 wks | N | N |
| Suryawanshi | 2018 | https://doi.org/10.1126/sciadv.aau4788 | Characterize cells from first-trimester maternal–fetal interface | RNA-seq | Single cell and bulk tissue | Chorionic villi and decidua | 6–11 wks | Y | N |
| Vento-Tormo | 2018 | https://doi.org/10.1038/s41586-018-0698-6 | Characterize cells from first-trimester maternal–fetal interface | RNA-seq | Single cell w/ pre-sorting (flow cytometry) | Chorionic villi and decidua | 6–14 wks | N | N |
| Turco | 2018 | https://doi.org/10.1038/s41586-018-0753-3 | Validate source and source-derived organoid | Microarray | Bulk tissue | Chorionic villi | 6–8 and 10–12 wks | N | N |
| Huang | 2018 | https://doi.org/10.1016/j.ebiom.2018.11.015 | Recurrent miscarriage | RNA-seq | Bulk tissue | Chorionic villi | < 20 wks | N | N |
| Zhao | 2018 | https://doi.org/10.19723/j.issn.1671-167X.2019.01.026 | IVF | Microarray | Bulk tissue | Chorionic villi | 7–8 wks | N | N |
| Gonzalez | 2019 | https://doi.org/10.1186/s13293-018-0165-y | Fetal sex differences | RNA-seq | Bulk tissue | Chorionic villi | 10.5–13.5 wks | Y | Y |
| Lee | 2019 | https://doi.org/10.1016/j.fertnstert.2018.11.005 | IVF and non-IVF treatment | RNA-seq | Bulk tissue | Chorionic villi | 11–13 wks | Y | N |
| Sun | 2020 | https://doi.org/10.1210/clinem/dgaa503 | Sexually dimorphic crosstalk at maternal–fetal interface | Single-cell and bulk RNA-seq | Single cell and bulk tissue | Chorionic villi and decidua | 10–13 wks | Y | Y |
| Braun | 2020 | https://doi.org/10.1007/s43032-020-00355-8 | Sex differences during fetal androgen exposure | RNA-seq | Bulk tissue | Chorionic villi | 11–16 wks | Y | Y |
| Flowers | 2021 | https://doi.org/10.1093/biolre/ioab221 |
Sex differences in microRNA expression in first and third trimester human placenta |
Small RNA-seq | Bulk tissue | Chorionic villi and placenta collected at delivery | 10.4–14.5 wks and 36.3–41.4 wks | Y | Y |
Bolded values indicate studies in which sex-based analysis is reported