FIG. 5.

Increased sensitivity of rpoE mutant V. angustum (EH1) to heat shock (A) and oxidative stress (B). (A) V. angustum wild-type (S141) (white bar) and rpoE mutant (EH1) (black bar) strains were grown to mid-log phase (OD₆₁₀) in MMM supplemented with glucose at 25°C and were shifted to 40°C. (B) V. angustum wild-type (S141) and rpoE mutant (EH1) cells were exposed to 65 μM H₂O₂ for 10 min at mid-log phase (S141, white bar; EH1, black bar) and exposed to 1 mM H₂O₂ for 60 min at both 0 h (S141, vertical stripes; EH1, horizontal stripes) and 24 h (S141, checks; EH1, diagonal stripes) of carbon starvation. Survival was assessed by viable counts of CFU and is expressed as the percentage of surviving cells relative to the initial cell viability. The results presented are mean values of three independent experiments, each carried out in triplicate. The error bars denote the standard deviation.