TABLE 2.

Primers used in this study^a

Primer	Sequence	Target sequence
1F	5′-GTCAAGGGATCACGGGTAGG-3′	rpoS on pRH324
2F	5′-AGTGCTGGCACGTCGATTCG-3′	rpoS on pRH324
1R	5′-AACACACGCTGTGTGGCTCC-3′	rpoS on pRH324
2R	5′-GCCTCGCTTGAGACTGGCCT-3′	rpoS on pRH324
C	5′-TCTAAGTATTCCTGAATCATTAAG-3′	rpoE on pEH8
F2.mut	5′-CCACAATATTAATGAGGTTTTAGG-3′	Upstream of rpoE on pEH8
F3.mut	5′-ACCTTCTAGCTTCACGCTGCCGC-3′	nptII on pBSL180
F6.mut	5′-GATGAATTGCCCGAAGATTAC-3′	rpoE on pEH8
F10	5′-ATGTATCGACTAAAAGCCGAG-3′	Downstream of rpoE in V. angustum S14
G	5′-GCATAGAAATGAATTAATTGAAGCG-3′	rpoE on pEH8
R2	5′-ATTATCTGTCGCTCTTTAATAGG-3′	Upstream of rpoE in V. angustum S14
R2.mut	5′-AAGGTGCTTTGCGCACTCTCTTT-3′	rpoE on pEH8
R4.mut	5′-ACCATAAACCCCAGAGTCCCGCTC-3′	nptII on pBSL180
R5.mut	5′-CTTTCAGGGCTGTGTATC-3′	Downstream of rpoE on pEH8

^aThe source for all primers is this study.