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(A) Proposed mechanism of singlet oxygen production without light excitation. The hydroxyl group on Schaap’s scaffold (highlighted in green) when deprotonated in PBS pH 7.4 causes dioxetane breakdown and green chemiluminescence. Due to the close proximity of the green-absorbing photosensitizer, Erythrosin B (highlighted in purple), chemiluminescence resonance energy transfer occurs, causing Erythrosin B to produce singlet oxygen. (B) Structures of control compounds.
